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T1192 Sigma

Anti-Thymine Dimer antibody, Mouse monoclonal

clone H3, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Cell Cycle,
biological source   mouse
antibody form   purified immunoglobulin
clone   H3, monoclonal
form   buffered aqueous solution
species reactivity   chicken, wide range
concentration   ~2 mg/mL
application(s)   capture ELISA: suitable
  dot blot: 0.5-1 μg/mL
  immunocytochemistry: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.

Other Notes

Distributed under license.
Patents WO87/01134, EP 0233 177 B1

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-Thymine Dimer antibody is suitable for use in dot blot (0.5-1 mg/mL), immunocytochemistry (1:100) and capture ELISA.

General description

UV light induces the formation of thymine dimers that subsequently leads to DNA damage in cells. Monoclonal antibodies that bind to thymine dimers enable the study of cell death and UV-induced DNA damage during photocarcinogenesis . Monoclonal Anti-Thymine Dimer binds to the (5’-6’) cyclobutane type of homothymine or thyminecytosine heterodimers in single-stranded DNA. The antibody has a decreased affinity for the dimer in short oligonucleotides (an oligonucleotide of atleast 10-20 thymine residues is required for the labeling of the probes). The product facilitates the use of a sensitive and non-radioactive technique for the analysis of thymine dimerization.

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