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T1299 Sigma

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-2, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurobiology,
species reactivity   human, monkey, rat, guinea pig, sheep, bovine, rabbit
application(s)   immunohistochemistry: suitable
  immunoprecipitation: suitable using native or SDS-denatured TH.
  western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
clone   TH-2, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TH(7054)
rat ... Th(25085)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rat tyrosine hydroxylase (TH).

General description

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Specificity

The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits.

Application

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson’s disease (PD).

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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06693 Timestrip Plus -20 °C

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