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T1410 Sigma

Trizma® base

cell culture tested, ≥99.9% (titration), crystalline

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 329826669

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Properties

Related Categories Biological Buffers, Cell Culture, Reagents and Supplements More...
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline
impurities   ≤0.2% water (Karl Fischer)
color   white
pH range   7.0 - 9.2
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   : soluble40 % (w/w), clear, colorless
cation traces   heavy metals (as Pb): ≤2 ppm
absorption   A40%/290 ≤0.05
suitability   cell culture tested

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
2
RTECS 
TY2900000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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T6066 Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

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