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T1410 Sigma

Trizma® base

cell culture tested, ≥99.9% (titration), crystalline

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 329826669

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Properties

Related Categories Biological Buffers, Cell Culture, Reagents and Supplements More...
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline
impurities   ≤0.2% water (Karl Fischer)
color   white
pH range   7.0 - 9.2
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   : soluble40 % (w/w), clear, colorless
cation traces   heavy metals (as Pb): ≤2 ppm
absorption   A40%/290 ≤0.05
suitability   cell culture tested

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
2
RTECS 
TY2900000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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T6066 Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

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