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T1503 Sigma

Trizma® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers, Buffers / Buffer salts, Buffers A to Z,
Quality Level   ELITE
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline
impurities   ≤0.2% water (Karl Fischer)
color   white
pH   10.5-12
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   water: soluble (678 g/l at 20 °C)
absorption   A40%/290 nm ≤0.05
Featured Industry   Diagnostic Assay Manufacturing

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

40% (w/w) solution in water is clear and colorless.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Go to Redi-Dri Product Listing RDD008

Packaging

1 kg in poly bottle

25, 100, 250, 500 g in poly bottle

5, 10, 25, 50 kg in poly drum

Application

Trizma base has been used-
• in carotenoid extraction from tubers
• along with Tween, for the saturation of microplate well prior to double sandwich ELISA (enzyme linked immunosorbent assay)
• as a component of sample buffer during protein extraction prior to Western blotting
• as a component of assay buffer during protein extraction prior to radioimmunoassay

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Ensure correct concentrations of buffer solutions with the Buffer Calculator

Price and Availability


Tris Always Available

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000
Protocols & Articles

Protocols

Elastase (EC 3.4.21.36) continuous spectrophotometric rate assay

The continuous spectrophotometric rate determination (A410, Light path = 1 cm) is based on the following reaction:
Keywords: Extinction coefficient

Enzymatic Assay and ATP Sensitivity Test of Luciferase

To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Keywords: Biochemistry, Enzyme activity, Enzymology, Size-exclusion chromatography

Enzymatic Assay of Achromopeptidase

The objective of this procedure is to standardize a method for the enzymatic assay of Achromopeptidase.
Keywords: Clinical, Microbiology

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.
Keywords: Hydration reaction, Phase transitions

Enzymatic Assay of Carboxypeptidase A

To standardize a procedure for the assay of Carboxypeptidase A at Sigma-Aldrich, St. Louis.
Keywords: Extinction coefficient

Enzymatic Assay of Chloramphenicol Acetyltransferase

To standardize a procedure for the determination of the enzymatic assay of Chloramphenicol Acetyltransferase.
Keywords: Enzyme activity, Enzymology, Extinction coefficient

Enzymatic Assay of Endoproteinase Glu-C (EC 3.4.21.19)

This procedure may be used for determination of Endoproteinase Glu-C enzymatic activity using N-t-Boc-L-glutamic acid α-phenyl ester as the substrate. This continuous UV spectrophotometric rate deter...
Keywords: Digestions, Enzyme activity, Extinction coefficient

Enzymatic Assay of Trypsinogen

This scope of this procedure applies to products that have a specification for the enzymatic activity of trypsinogen.
Keywords: Enzyme activity, Extinction coefficient

Procedure for Enzymatic Assay of Chymotrypsin (α‑Chymotrypsin, EC 3.4.21.1)

This procedure is for products with a specification for Chymotrypsin activity. It is not to be used to assay Insoluble Chymotrypsin such as Catalog No. C9134. The procedure is a continuous spectropho...
Keywords: Extinction coefficient

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Western Blotting (Immunoblotting) Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Buffer Reference Center

Citric Acid – Na2HPO4 Buffer Solutions, pH approx. 2.6–7.61 Citric acid monohydrate, C6H8O7 • H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.4...
Keywords: Biological Buffers, Buffers

Peer-Reviewed Papers
15

References

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