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  • T1815 - Monoclonal Anti-Tumor Necrosis Factor Receptor II antibody produced in mouse

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T1815 Sigma

Monoclonal Anti-Tumor Necrosis Factor Receptor II antibody produced in mouse

clone 22221, purified immunoglobulin, lyophilized powder

Synonym: Anti-TNF RII, Anti-TNFRSF1B

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   immunocytochemistry: 8-25 μg/mL using immersion fixed human peripheral blood lymphocytes
  microarray: suitable
  neutralization: suitable
clone   22221, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2a
storage temp.   −20°C
Gene Information   human ... TNF(7124)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human tumor necrosis factor receptor II, expressed in Escherichia coli.

General description

The tumor necrosis factor super family (TNFSF) consists of at least 19 transmembrane proteins with soluble forms that are generated by proteolysis. The biological effects of TNF are mediated by either type I or type II receptors. Type I receptors that are ubiquitously present on all cells whereas type II receptors are expressed only on cells of immune system (monocytes, macrophages, Langerhans cells) and endothelial cells. Receptors of TNFSF such as TNFR I and Fas have their own death domains that activate the apoptosis. Other receptors such as TNFR II do not have death domains and utilize TNF associated factors (TRAFs) to activate intracellular signaling. TNFR II activates NF-κB pathway via the activation of NIK. Monoclonal Anti-Tumor Necrosis Factor Receptor II antibody recognizes human TNFR II.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Specificity

The antibody neutralizes the biological effect of recombinant human TNF RII. It does not neutralize or cross-react with TNF RI.

Preparation Note

Purified using protein A

Application

Anti-Tumor Necrosis Factor Receptor II antibody may be used for immunocytochemistry at a working concentration of 8-25 μg/ml using human peripheral blood lymphocytes. The antibody is suitable for neutralization assays and protein microarray. The antibody was used for flow cytometry using human CD8 T cells.

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