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T1816 Sigma

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNF-α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   pig
application(s)   immunohistochemistry: 15 μg/mL
  indirect ELISA: 0.5-1.0 μg/mL
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   pig ... TNF(397086)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant porcine TNF-α expressed in Escherichia coli.

General description

Tumor Necrosis Factor-α (TNF-α) also refers as cachectin is a cytokine produced in response to immunological challenges such as bacteria (lipopolysaccharides), parasites, mitogens, viruses and other cytokines. It has essential roles in antitumor activity, inflammation, septic shock, immune modulation, anorexia, cachexia, hematopoiesis and viral replication. It also facilitates the growth of human fibroblasts, collagenase production as well as can activates polymorphonuclear neutrophils and osteoclasts. Anti-Tumor Necrosis Factor-α antibody can be used in neutralization and immunoblotting (working concentration-0.1-0.2 μg/ml) for detecting porcine TNF-α. Goat anti-Tumor Necrosis Factor-α antibody reacts specifically with recombinant porcine tumor necrosis factor α.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline.

Specificity

The antibody neutralizes the biological activity of recombinant porcine TNF-α.

Application

Anti-Tumor Necrosis Factor-α antibody can be used in immunohistochemistry and indirect ELISA.

Price and Availability


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