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T1895 Sigma

Thymidine

powder, BioReagent, suitable for cell culture

Synonym: 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT

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Properties

Related Categories Cell Culture, Conditioned Media, Media Supplements and Reagents, Hybridoma Reagents, Miscellaneous Reagents and Supplements, Reagents and Supplements More...
InChI Key   IQFYYKKMVGJFEH-XLPZGREQSA-N
product line   BioReagent
assay   ≥99%
form   powder
mp   186-188 °C(lit.)
suitability   suitable for cell culture
Gene Information   human ... DTYMK(1841)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Use in HT and HAT cocktails for hybridoma fusion, selection and cloning.

Packaging

1, 5, 10, 25 g in poly bottle

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
XP2071000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How can I solubilize Product T1895, Thymidine?
This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.
What concentration of Product T1895, Thymidine, is used for testing this product for cell culture applications?
The tested working concentration for cell culture for Thymidine is 8 mg/L.
How can I sterilize a solution of Product T1895, Thymidine?
Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.
How long can I store media supplemented with Product T1895, Thymidine?
Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L.  This media has an expiration date of 18 months from date of formulation.  You will need to determine if this is appropriate for your application.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
15

References

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