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T2663 Sigma

Trizma® hydrochloride solution

pH 7.4, 1 M, BioReagent, for molecular biology

Synonym: Tris hydrochloride solution

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
  for molecular biology
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
sterility   0.2 μm filtered
product line   BioReagent
concentration   1 M
impurities   DNase, RNase, NICKase and protease, none detected
pH   7.4
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

General description

Trizma® hydrochloride solution is a useful biological buffer.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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