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T2692 Sigma

Anti-TRANCE antibody produced in goat

affinity isolated antibody, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   mouse
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   mouse ... Tnfsf11(21943)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant mouse TRANCE (TNF-related activation induced cytokine), expressed in NSO cells.

General description

TRANCE, also called RANKL (RANK ligand), OPGL (Osteoprotegrin ligand), and ODF (Osteoclast differentiation factor) is a member of tumor necrosis factor superfamily that is expressed on the surface of osteoblasts and is released by activated T cells. When TRANCE binds to its receptor RANK it stimulates the formation, activation, survival and adherence of osteoclasts that increases the bone resorption. These effects are inhibited when RANKL binds to OPG that leads to decrease in bone resorption process and increase in bone volume and density. The TRANCE (RANKL)/RANK/OPG collectively activates NF-κB, JNK, Akt, p38, ERK, NFATc1 pathways. This axis is vital for maintaining the bone density, dendritic cell survival, B cell differentiation, mammary gland development. This signalling pathway maintains the osteoclast biology and is a therapeutic target for osteoporosis
Anti-TRANCE (RANKL) recognizes recombinant mouse TRANCE (TNF-related activation-induced cytokine). This antibody demonstrates no cross reactivity with recombinant human CD40 ligand.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Specificity

The antibody neutralizes the biological activity of recombinant mouse TRANCE. By immunoblotting, this antibody shows no cross-reactivity with recombinant human CD40 ligand.

Application

Anti-TRANCE antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA a concentration of 0.5-1.0 μg/ml is recommended. The antibody is also suitable for neutralization reactions (ND50 is 0.02-0.06 μg/ml).

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