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T2780 Sigma

Monoclonal Anti-Tropomyosin antibody produced in mouse

clone TM311, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   rabbit, pig, bovine, human, rat, chicken, mouse, hamster
application(s)   immunoprecipitation: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:400 using chicken fibroblasts
  microarray: suitable
  western blot: suitable using human tissue and chicken gizzard extracts
clone   TM311, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 36-39 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   bovine ... TPM1(281544)
chicken ... TPM1(396366)
human ... TPM1(7168)
mouse ... Tpm1(22003)
rat ... Tpm1(24851)
biological source   mouse
conjugate   unconjugated



chicken gizzard tropomyosin.

General description

Tropomyosin is a rigid rod shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. Tropomyosin together with troponin, regulate the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Varoius tropomyosin isoforms help to confer structure and function to actins in the cytoskeleton and in striated muscle function.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunohistochemistry-frozen tissue (1 paper)

Mouse monoclonal clone TM311 anti-Tropomyosin antibody is used to tag tropomyosin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of tropomyosin in cytoskeletal structures.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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