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T2817 Sigma

Anti-Tie 1 antibody produced in goat

affinity isolated antibody, lyophilized powder

Properties

Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... TIE1(7075)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human Tie-1 extracellular domain expressed in NSO cells.

General description

Tie-1 and Tie-2 belong to the Tie (Tyrosine kinase with Immunoglobulin-like and EGF homology) family of receptor tyrosine kinases. The expression of Tie-1 is exclusive to endothelial cells and cells of hematopoietic lineage. It is expressed in lung, brain, heart, liver and kidneys during the development of embryo. It is essential for ensuring the integrity of fetal vasculature. The expression of Tie-1 is critical for the maintenance of shear stress in blood vessels. Overexpression of Tie-1 is observed in inflammatory tissue of rheumatoid arthritis, atherosclerosis and osteoarthritis patients. In response to inflammation Tie-1 activates the NF-κB and PI3K pathways and increases the expression of VCAM-1 and ICAM-1 proteins
Anti-Tie-1 recognizes human Tie-1. This antibody exhibits ~1% cross-reactivity with recombinant human Tie-2.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Specificity

The antibody demonstrates approximately 1% cross-reactivity with recombinant human Tie-2.

Application

Anti-Tie-1 antibody may be used for immunoblotting at a working antibody concentration of 0.1-0.2 μg/ml. For ELISA, a working antibody concentration of 0.5-1.0 μg/ml is recommended.

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