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T2824 Sigma

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-TNF-α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Tumor Necrosis Factor (TNF) Superfamily,
species reactivity   mouse, rat
application(s)   immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  neutralization: suitable
  western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α
clone   TN3-19.12, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Tnf(21926)
rat ... Tnf(24835)
conjugate   unconjugated

Description

Immunogen

purified recombinant mouse TNF-α.

General description

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.

TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

Physical form

Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Application

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody is a useful tool for the determination and quantification of the TNF-α molecule in many in vitro and in vivo systems. The product may be used in various immunochemical techniques such as immunoblotting, ELISA, immunoprecipitation, and neutralization.

Price and Availability


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