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T3038 Sigma

Trizma® hydrochloride solution

pH 8.0, 1 M, BioReagent, for molecular biology

Synonym: Tris hydrochloride solution

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
  for molecular biology
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
sterility   0.2 μm filtered
product line   BioReagent
concentration   1 M
impurities   DNase, RNase, NICKase and protease, none detected
pH   8.0
Featured Industry   Agriculture
Diagnostic Assay Manufacturing

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma® hydrochloride solution may be used in the following studies:
• As buffer for the 2-D electrophoresis of rat fibroblast cell.
• As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
• Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

General description

Trizma® hydrochloride solution is a useful biological buffer.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

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Peer-Reviewed Papers
15

References

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