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T3175 Sigma

Monoclonal Anti-Terminal Deoxynucleotidyl Transferase

clone 8-1 E4, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-TdT

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies to DNA Synthesis and Repair Enzymes, Antibodies to Human Leukocytes,
species reactivity   bovine, human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
clone   8-1 E4, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C
Gene Information   bovine ... DNTT(281120)
human ... DNTT(1791)
conjugate   unconjugated

Description

Immunogen

bovine TdT.

Antigen Background

TdT is a non-template directed DNA polymerase that catalyzes the irreversible addition of deoxynucleotides to the 3′-hydroxy groups found on the end of DNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Reacts with the lymphoblast cell line 8402 and not with normal human peripheral lymphocytes as determined by immunohistology and flow cytometry. It is specific for terminal deoxynucleotidyl transferase as determined by ELISA with bovine TdT. Anti-TdT is a useful marker for immature and neoplastic cells frequently found in leukemic states.

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Safety Information

WGK Germany  3
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