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T3176 Sigma

Anti-Tumor Necrosis Factor-β antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-TNF-β

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Description

Immunogen

human recombinant TNF-β.

General description

Tumor necrosis factors are important inflammatory cytokines and modulate the immune responses. TNF-α and TNF-β are two closely related cytokines. TNF-β is also known as lymphotoxin-α or LT-α and is produced by T lymphocytes in response to antigenic or mitogenic stimulation. TNF-α and TNF-β show 30% homology but also have different biological and molecular functions. TNF-β is not found on the cell surface but is bound to the cell membrane in association with LT β and binds strongly to TNFR1 and TNFR2 receptors. TNF-β is expressed by B cells, CD4+ cells, CD8+ cells, macrophages and natural killer cells. Studies indicate the important role of TNF-β in the development of lymphoid organs, maintenance of lymphoid environment, host defense responses against certain infections caused by Mycobacterium-italics, Staphylococcus-italics, Leishmania-italics and Plasmodium-italics. Increased levels of TNF-β have been observed in patients suffering from rheumatoid arthritis
Anti-Tumor Necrosis Factor-β antibody specifically reacts and neutralizes human TNF-β. It does not neutralize the biological activity of either human or mouse TNF-β.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Specificity

The antibody will neutralize the biological activity of recombinant human TNF-β.

Preparation Note

Purified using protein G.

Application

Anti-Tumor Necrosis Factor-β antibody may be used to neutralize the biological activity of recombinant or natural human TNF-β at a final concentration of 1 ng/ml. The antibody may be used at a working concentration of 1.0 μg/ml for indirect ELISA and at 2 μg/ml concentration for immunoblotting.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
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