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T3194 Sigma

Tetanus toxin from Clostridium tetani

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Properties

Related Categories Bacteria, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience,
mol wt   mol wt 150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
solubility   H2O: soluble
storage temp.   2-8°C
Gene Information   Clostridium tetani E88 ... tetX(1061100)

Description

Analysis Note

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

General description

Each lot of tetanus toxin is tested to confirm binding activity to GT1b ganglioside using the hemagglutination assay of Tayot, et al.

Preparation Note

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

Reconstitution

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

Application

Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.

Biochem/physiol Actions

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
RIDADR 
UN3462 - class 6.1 - PG 1 - Toxins, extracted from living sources, solid, n.o.s., HI: all (not BR)
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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