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T3253 Sigma

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   ELITE
grade   reagent grade
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
assay   ≥99.0% (titration)
form   crystalline
impurities   ≤0.5% water (Karl Fischer)
color   white
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble clear, colorless
absorption   A40%/290 nm ≤0.05
Featured Industry   Diagnostic Assay Manufacturing

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 kg in poly bottle

100, 250, 500 g in poly bottle

5, 10, 25, 50 kg in poly drum

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

General description

Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD009

Price and Availability


Added Consistency

Tris Always Available

Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the pH of Trizma HCl, Product T3253, in solution?
The pH of Trizma hydrochloride in water is dependent on the concentration. The pH of a 0.1 M aqueous solution is approximately 4.7 while the pH of a 40% (w/w) aqueous solution is typically 2.6- 3.3. This information can be found on the product information sheet at the following link:http://www.sigmaaldrich.com/sigma/product%20information%20sheet/t3253pis.pdf
How can I find out more information about other biological buffers?
Useful information on buffers (including Trizma buffer) can be found using the Buffer Explorer on the Sigma Aldrich website at the following link:http://www.sigmaaldrich.com/Area_of_Interest/Biochemicals/Buffer_Explorer.html
How can I find the technical bulletin 106B that is referenced in the Product Information Sheet for Product T3253, Trizma® hydrochloride?
The following is a direct link to the file:http://www.sigmaaldrich.com/sigma/bulletin/106bbul.pdf
Is Product T3253, Trizma® hydrochloride, the same as Tris buffer?
No. Trizma HCl in solution at 0.05 M will produce a pH of approximately 4.7, but will have little if any buffering capacity. Trizma Base in solution at 0.05 M will produce a pH of approximately 10.4, but will have little if any buffering capacity. Blending Trizma Base and Trizma HCl will produce any desired pH between 7 and 9, with a reasonable buffering capacity.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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