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T3253 Sigma

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   ELITE
grade   reagent grade
InChI Key   QKNYBSVHEMOAJP-UHFFFAOYSA-N
assay   ≥99.0% (titration)
form   crystalline
impurities   ≤0.5% water (Karl Fischer)
color   white
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble, clear, colorless
absorption   A40%/290 nm ≤0.05
Featured Industry   Diagnostic Assay Manufacturing

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 kg in poly bottle

100, 250, 500 g in poly bottle

5, 10, 25, 50 kg in poly drum

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD009

Price and Availability


Tris Always Available

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Related Content

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A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
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