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T3287 Sigma

Monoclonal Anti-Talin antibody produced in mouse

clone 8d4, ascites fluid

Synonym: Monoclonal Anti-Talin

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell Membrane,
species reactivity   frog, mouse, rat, human, chicken
application(s)   immunoprecipitation: suitable
  indirect immunofluorescence: 1:500 using cultured Rat2 cells
  western blot: 1:100 using chicken gizzard extract
clone   8d4, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 225-235 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TLN1(7094), TLN2(83660)
mouse ... Tln1(21894), Tln2(70549)
rat ... Tln1(313494)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified chicken gizzard talin.

Specificity

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts.

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