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T3320 Sigma

Anti-γ-Tubulin (AK-15) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Centrosome,
species reactivity   frog, rat, human
application(s)   immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells
  microarray: suitable
  western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line.
  western blot: 1:5,000 using Xenopus eggs extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 48 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBG1(7283), TUBG2(27175)
rat ... Tubg1(252921), Tubg2_predicted(360633)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of Xenopus γ-tubulin (amino acids 437-451 with N-terminal added cys-gly), conjugated to KLH.

General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:
• Western blot.
• Immunofluorescence.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

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