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T3405 Sigma

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

tablet, 1 mg substrate per tablet

DOWNLOAD MSDS (PDF)

Synonym: 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride tablet, 1 mg substrate per tablet
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Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

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