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T3413 Sigma

Monoclonal Anti-Tenascin antibody produced in rat

clone MTn-12, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   mouse
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:200 using unfixed, frozen tissue sections of mouse intestine
  western blot: suitable
clone   MTn-12, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Tnn(329278)
biological source   rat
conjugate   unconjugated

Description

Immunogen

partially purified mouse tenascin.

General description

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).
The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine.
Human tenascin has 3 subunits of 190, 200 and 220 kDa. Tenascin has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.

Specificity

The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-Mouse Tenascin antibody may be used for the localization of tenascin and to study of the role of tenascin in epithelial-mesenchymal interactions using various immunochemical assays including ELISA, immunoblot, dot blot and immunohistology.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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