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T3526 Sigma

Anti-Tubulin antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   mouse, chicken
application(s)   indirect immunofluorescence: 1:80 using cultured fibroblasts
  western blot: 1:200 using chicken gizzard extract or mouse brain extract
clone   polyclonal
antibody form   whole antiserum
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Tuba1b(22143), Tubb2a(22151), Tubb2b(73710), Tubb2c(227613)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Specificity

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Application

Anti-Tubulin antibody is qualified for indirect immunofluorescence, a working dilution of at least 1:80 using cultured fibroblasts and Anti-Rabbit IgG-FITC (Product No. F9887) as the secondary antibody and immunoblotting, a working dilution of at least 1:200 using chicken gizzard extract or mouse brain extract and Anti-Rabbit IgG-Peroxidase (Product No. A0545) as the secondary antibody.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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