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T3819 Sigma

Tetanus toxin C-fragment−FITC from Clostridium tetani

Synonym: TTC-FITC

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Properties

Related Categories Bacteria, Cell Biology, Cell Signaling and Neuroscience, Glycinergics, Neuroscience,
solubility   H2O: soluble
storage temp.   2-8°C
Gene Information   Clostridium tetani E88 ... tetX(1061100)

Description

General description

The μg amount of FITC bound per mg of C-fragment is determined assuming an extinction coefficient at 493 nm of 85,200 M-1cm-1 for FITC

binding activity to GT1b gangliosides has been confirmed by the hemagglutination assay of Tayot, et al.

Preparation Note

Prepared by conjugating the C-fragment of tetanus toxin to fluorescein isothiocyanate, isomer I (FITC) by a modification of the method of Wood et al.

Reconstitution

When reconstituted with 0.125 ml of sterile water, each vial contains 25 μg of protein in 0.01 m sodium phosphate, pH 7.5. Protect from light.

Biochem/physiol Actions

C terminal portion conjugated to fluorescein isothiocyanate is taken up by persynaptic nerve terminals and exhibits transsynaptic retrograde transport in central neurons similar to that of tetanus toxin. It is used to map neuronal connections and to target proteins to neurons

Tetanus toxin is a neurotoxin that comprises of a heavy and a light chain linked by disulfide bridges. This neurotoxin enters the cytoplasm and releases the light chain component that subsequently inhibits the release of neurotransmitters3. Studies have reported that the fragment C of tetanus toxin retains most of the determinants that interact with thyroid membranes, gangliosides and neural membranes4.

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All labs need water
Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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