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T3924 Sigma

Trypsin-EDTA solution

1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

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Description

Components

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Biochem/physiol Actions

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Caution

This product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.

Preparation Note

This product does contain phenol red. Due to shipment on dry ice, there could be significant carbon dioxide buildup in the package. This CO2 may enter the solution and lower the pH slightly, giving an orange rather than pinkish color. The orange solution will still be suitable for use, or the pH can be adjusted with sodium hydroxide. Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containing no Ca2+ or Mg2+.

Application

The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

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Safety & Documentation

Safety Information

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Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Protocols

Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Aortic Smooth Muscle Cells (BAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Brain Artery Endothelial Cells (BBAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Brain Microvascular Endothelial Cells (BBMVEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Coronary Artery Endothelial Cells (BCAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Coronary Artery Smooth Muscle Cells (BCASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Endometrial Endothelial Cells (BEnEpC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Pulmonary Artery Endothelial Cells (BPAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Bovine Renal Artery Smooth Muscle Cells (BRASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Aortic Endothelial Cells (CnAOEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Aortic Smooth Muscle Cells (CnAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Chondrocytes (CnC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Coronary Artery Smooth Muscle Cells (CnCASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Marrow Stromal Cells (CnMSC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Osteoblasts (CnOb) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Pulmonary Artery Smooth Muscle Cells (CnPASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Canine Skeletal Muscle Cells (CnSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Feline Chondrocytes (FC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Feline Marrow Stromal Cells (FMSC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Feline Osteoblasts (FOb) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Feline Skeletal Muscle Cells (FSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Aortic Smooth Muscle Cells (HAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Astrocytes (HA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Brachiocephalic Artery Endothelial Cells (HBcAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Bronchial Epithelial Cells (HBEpC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Cardiac Fibroblasts (HCF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Carotid Artery Endothelial Cells (HCtAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Carotid Artery Smooth Muscle Cells (HCtASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Chondrocytes (HC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Chondrocytes Osteoarthritis (HC-OA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Chondrocytes Rheumatoid Arthritis (HC-RA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Coronary Artery Smooth Muscle Cells (HCASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Dermal Fibroblasts (HDF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Dermal Microvascular Endothelial Cells (CADMEC™/HMVEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Epidermal Keratinocytes (HEK) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Epidermal Melanocytes (HEM) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Fibroblast-Like Synoviocytes Osteoarthritis (HFLS-OA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Internal Thoracic Artery Endothelial Cells (HITAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Internal Thoracic Artery Smooth Muscle Cells (HITASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Lung Fibroblasts (HLF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Mammary Epithelial Cells (HMEpC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Marrow Stromal Cells (HMSC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Osteoblasts (HOb) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Osteoblasts Osteoarthritis (HOb-OA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Osteoblasts Rheumatoid Arthritis (HOb-RA) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Placental Epithelial Cells (HPlEpC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Preadipocytes (HPAd) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Pulmonary Artery Endothelial Cells (HPAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Subclavian Artery Endothelial Cells (HScAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Subclavian Artery Smooth Muscle Cells (HScASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Tracheal Epithelial Cells (HTEpC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Umbilical Artery Endothelial Cells (HUAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Umbilical Artery Smooth Muscle Cells (HUASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Human Umbilical Vein Smooth Muscle Cells (HUVSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Aortic Endothelial Cells (PAOEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Aortic Smooth Muscle Cells (PAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Coronary Artery Endothelial Cells (PCAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Coronary Artery Smooth Muscle Cells (PCASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Pulmonary Artery Endothelial Cells (PPAEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Porcine Pulmonary Artery Smooth Muscle Cells (PPASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

0.1 Seed 15k MEF per well into 96-well plate. Utilize a final volume of 200 µl of media per well. (Note: for best data interpretation always include an untransduced well that is treated with puromyci...

Rabbit Aortic Smooth Muscle Cells (RbAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rabbit Skeletal Muscle Cells (RbSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Aortic Endothelial Cells (RAOEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Aortic Smooth Muscle Cells (RAOSMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Cardiac Fibroblasts (RCF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Dermal Fibroblasts (RDF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Epidermal Keratinocytes (REK) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Lung Fibroblasts (RLF) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Marrow Stromal Cells (RMSC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Osteoblasts (ROb) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Pulmonary Artery Smooth Muscle Cells (RPASMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Rat Skeletal Muscle Cells (RSkMC) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Removal of Adherent Cells from Culture Surface Using Trypsin

Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin or trypsin/EDTA solutions. Trypsin concentrat...

Peer-Reviewed Papers
15

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