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T4019 Sigma

Trypsin−Agarose

buffered aqueous suspension, trypsin from bovine pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Dissociation, Cell Dissociation and Cell Lysis, Enzymes, Inhibitors, and Substrates,
biological source   trypsin from bovine pancreas
form   buffered aqueous suspension
extent of labeling   ≥15 units per mL (packed gel)
matrix   beaded agarose
storage temp.   2-8°C

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Preparation Note

TPCK-treated

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2, containing 0.01% thimerosal

Application

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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