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T4026 Sigma

Monoclonal Anti-β-Tubulin antibody produced in mouse

clone TUB 2.1, ascites fluid

Synonym: Monoclonal Anti-β-Tubulin

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins,
species reactivity   chicken, human, frog, wheat, plant, rabbit, bovine, mouse, hamster, moth, rat, sea urchin
application(s)   indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
  western blot: 1:100 using human or chicken fibroblast cell extract
clone   TUB 2.1, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 55 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

tubulin from rat brain.

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules.β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Application

Monoclonal Anti β-Tubulin antibody produced in mouse has been used in following studies:
• Fluorescence-activated cell sorting (FACS).
• Immunoblotting.
• Immunofluorescence Analysis.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β- 1 tubulin also known as Class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

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