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T4376 Sigma

N-p-Tosyl-L-phenylalanine chloromethyl ketone

≥97% (TLC), powder

Synonym: (S)-1-Chloro-3-tosylamido-4-phenyl-2-butanone, (S)-1-Chloro-4-phenyl-3-tosylamido-2-butanone, TPCK, Tosyl-L-phenylalanyl-chloromethane

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Properties

Related Categories A to C, Biochemicals and Reagents, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes, Bromelain, Cell Biology,
InChI Key   MQUQNUAYKLCRME-INIZCTEOSA-N
assay   ≥97% (TLC)
form   powder
potency   10-100 μM effective concentration
color   white
mp   106-108 °C(lit.)
solubility   DMSO: >10 mg/mL (stable for several months at 4°C.)
  ethanol: soluble20 mg/mL (stable for several months at 4°C. Stock solutions (10 mM in methanol or ethanol) are stable for several months at 4°C.)
storage temp.   −20°C
Gene Information   human ... NFKB1(4790), NKAP(79576), NKRF(55922), NOS2(4843), PTGS2(5743)

Description

Application

Irreversible inhibitor of certain serine proteases; used to inhibit chymotrypsin in preparations of trypsin.

Caution

Shelf-life is at least two years when stored desiccated at -20 °C.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Biochem/physiol Actions

Blocks the LPS- or cytokine-induced activation of nuclear factor κB (NFκB), which, in turn, blocks the induction of iNOS and COX-2 transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli. Also blocks apoptosis initiated by Taxol (in MCF-7 human breast carcinoma cells).

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
XT5613500
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Description

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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