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T4427 Sigma

Terminal Transferase from calf thymus

buffered aqueous glycerol solution

Synonym: TdT, Terminal Deoxynucleotidyl Transferase




Terminal transferase is supplied as a solution of 50 mM potassium phosphate (pH 7.4), 1 mM 2-mercaptoethanol and 50% glycerol (v/v).

Analysis Note

Activity assay uses 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C.

General description

Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present.

Unit Definition

One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer.


Suitable for:
• Addition of homopolymers to vectors, inserts and cDNA for cloning
• Labeling the 3′-end of double- and single-stranded DNA with non-radioactive or radioactive labels
• Carrying out in vitro mutagenesis by adding single nucleotides to DNA
• Use in TUNEL assays

Price and Availability

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Terminal Transferase from calf thymus

Product Description Precautions and Disclaimer Storage/Stability Procedure Materials References
Keywords: Buffers, Cloning

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