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T4444 Sigma

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA

ready to use solution

Synonym: TMB

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Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Protocols & Articles

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Antibody Basics

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

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BioFiles 2008, 3.4, 6.
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