T4532 Sigma

Tryptose Broth

insect cell culture tested



Related Categories Bacterial Culture Media, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology, Tryptose Broth
suitability   insect cell culture tested
storage temp.   2-8°C



Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Other Notes

This Tryptose broth is closely related to Tryptose phosphate broth, but does not contain the phosphate component and it contains a lower concentration of dextrose.


1 kg in poly bottle

250 g in poly bottle

Preparation Note

This formulation contains Tryptose (20 g/L); Dextrose (1 g/L); and NaCl (5g/L) adjusted to a final pH of 7.2.


In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers


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