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T4817 Sigma

Anti-Transforming Growth Factor-β5 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-TGF-β5

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Description

Immunogen

recombinant amphibian transforming growth factor-β5 expressed in Sf 21 cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Application

Anti-TGF-β5 antibody is suitable for neutralization reactions (ND50 is 0.03-0.08 μg/ml). The antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 1 μg/ml is recommended.

General description

Transforming growth factor-β5 (TGF-β) belongs to the TGF-β superfamily of secreted proteins. The TGF-β family includes five isoforms, TGF-β1 toTGF-β5. The isoforms are involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β5 isoform is expressed in the embryonic as well as adult stages of Xenopus. TGF-β5 is involved in cell and tissue differentiation and proliferation. TGF-β5 expression is not observed in mammals
Anti-TGF-β5 recognizes the amphibian TGF-β5. It does not show cross reactivity with TGF-β1, porcine TGF-β2, TGF-β2, or TGF-β3.

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Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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