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T5168 Sigma

Monoclonal Anti-α-Tubulin antibody produced in mouse

ascites fluid, clone B-5-1-2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human, Chlamydomonas, African green monkey, chicken, mouse, bovine, rat, kangaroo rat, sea urchin
application(s)   indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts
  radioimmunoassay: suitable
  western blot: 1:4,000 using human fibroblast cell extract
clone   B-5-1-2, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

General description

α -tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:
In immunofluorescence Analysis.
In western blotting/ Immunoblotting.
For immunolabelling cells in electron microscopy.

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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