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T5192 Sigma

Anti-γ-Tubulin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-γ-Tubulin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Centrosome,
species reactivity   chicken, human
application(s)   indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblasts cells
  western blot: 1:1,000 using whole cell extract human epidermal carcinoma A431 cell line
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 48 kDa
storage temp.   −20°C
Gene Information   human ... TUBG1(7283), TUBG2(27175)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulinγ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in following studies:
• Immunocytochemistry.
• Immunofluorescence.
• Immunoblotting.
• Staining in microscopy.
• Chromatin immunoprecipitation (ChIP).

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

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