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T5293 Sigma

Monoclonal Anti-β-Tubulin antibody produced in mouse

clone 2-28-33, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins,
species reactivity   rat, chicken, bovine, Caenorhabditis elegans, Strongylocentrotus purpuratus, Ciona intestinalis, human
application(s)   indirect immunofluorescence: 1:1,000 using cultured chicken fibroblasts or cultured human foreskin fibroblasts
  western blot: 1:100
clone   2-28-33, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
rat ... Tubb2(29212), Tubb2c(296554)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Specificity

The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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