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T5319 Sigma

Tetanolysin from Clostridium tetani

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Description

Analysis Note

Single band by SDS-PAGE.

Preparation Note

Prepared by a modification of the method of Haque, et al.

Reconstitution

When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.

Application

Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)1. It has also been used to permeabilize infected RBCs2.

Biochem/physiol Actions

Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.

General description

Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

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Peer-Reviewed Papers
15

References

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