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T5326 Sigma

Monoclonal Anti-γ-Tubulin antibody produced in mouse

clone GTU-88, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Centrosome,
species reactivity   Xenopus, canine, chicken, hamster, human, rat, bovine, mouse
application(s)   immunocytochemistry: 1-2 μg/mL using HeLa cells
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts
clone   GTU-88, monoclonal
concentration   ~1 mg/mL
antibody form   purified antibody (purified from hybridoma cell culture)
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 48 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)
biological source   mouse

Description

Immunogen

synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH.

General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Application

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:
• Western blot analysis.
• Immunohistochemistry.
• Immunocytology.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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