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T5393 Sigma

Anti-Mouse IgG (whole molecule)−TRITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct immunofluorescence: 1:64
conjugate   TRITC conjugate
storage temp.   2-8°C

Description

Immunogen

IgG isolated from pooled normal mouse serum

Packaging

0.5 mL in glass insert

1, 2 mL in glass bottle

Specificity

The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-Mouse IgG (whole molecule)-TRITC antibody may be used for immunofluorescence at a minimum antibody dilution of 1:64 using mouse spleen cells. Antibody dilution range of 1:50 to 1:250 was used for immunofluorescence in various studies.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Antibody purification is by ion exchange chromatography after which it is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC).

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. The antibody is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC. Specificity for mouse IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for mouse IgG and all subclasses, it cross reacts with mouse IgA and IgM. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the γ region.

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Safety Information

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GHS08  GHS08
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Warning
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NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Peer-Reviewed Papers
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