T5525 Sigma

3,3′,5,5′-Tetramethylbenzidine

tablet, 1 mg substrate per tablet

DOWNLOAD MSDS (PDF)

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8

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