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  • T5817 - Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse

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T5817 Sigma

Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse

~1 mg/mL, clone 6H5-3, purified immunoglobulin, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Hematopoietic Stem Cells,
species reactivity   mouse, human
application(s)   immunohistochemistry: suitable using 4% formaldehyde, paraffin-embedded human and mouse tissue
  immunoprecipitation: suitable
  western blot: 1-4 μg/mL using whole extracts of Cos-1 cells transfected with human TCF-4
clone   6H5-3, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous glycerol solution
isotype   IgG2a
mol wt   antigen mol wt 66 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... TCF7L2(6934)
mouse ... Tcf7l2(21416)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

His-tagged fusion protein corresponding to amino acids 31-331 of human TCF-4

General description

Transcription factor 4 or T-Cell Factor-4 (TCF-4), also called as transcription factor 7 like 2 (TCF7L2), is mapped to human chromosome 18q21.1. The gene codes for a basic helix-loop-helix DNA binding protein, which belongs to TCF family and is implicated in transcriptional regulation. It is expressed predominantly in nervous system. TCF4 protein has a transcriptional activation domain 2 (AD2), a transcriptional activation domain (AD1) and a nuclear localization signal (NLS) in the N-terminal end and a C-terminal basic helix-loop-helix (bHLH) domain, involved in dimerization and deoxyribonucleic acid (DNA) binding.

Physical form

Solution in 0.7 M tris-glycine, pH 7.4, containing 0.105 M sodium chloride, 30% glycerol and 0.035% sodium azide.

Application

May be used for gel-shift assays

Biochem/physiol Actions

Transcription factor 4 (TCF-4) interacts functionally with β-catenin to mediate Wnt signaling pathway and transactivates downstream target genes implicated in cancer development. TCF-4 plays a vital role in development of the nervous system and vertebrate development. Aberrations or mutation of the gene leads to Pitt-Hopkins syndrome (PTHS), a rare developmental disorder characterized by severe motor and mental retardation, typical facial features and breathing abnormalities.

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Safety Information

Personal Protective Equipment  Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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