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T5820 Sigma

Tissue protein, cell membrane

from human skeletal muscle

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Description

Application

Highly purified cell membrane protein from whole tissue homogenates. Useful in electrophoresis, Western blotting, immunoassay, and other applications requiring source-specific, non-denatured protein.

Physical form

0.5 mg protein (approx. 3 mg protein/mL)

Supplied in a solution containing 20 mM HEPES, 1.5 mM MgCl2, 10 mM KCl, 40 mM sucrose, 1 mM EDTA, 2% glycerol, 0.5% sodium deoxycholate, 1% NP-40, pH 7.9, and a cocktail of protease inhibitors.

Price and Availability


Amplified Detection

Ultrafree Centrifugal Filter Units
Safety & Documentation

Safety Information

WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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