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T5912 Sigma

Tris Buffered Saline

10 ×, solution

Synonym: TBS

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z More...
sterility   0.2 μm filtered
form   solution
concentration   10 ×
storage temp.   2-8°C

Description

Other Notes

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl

Application

Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.

Suitability

Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

Price and Availability


Available in ELITE Grade

Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Protocols

4-Chloro-1-Naphthol Solution Protocol

Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotti...
Keywords: Western blot

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

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