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T5941 Sigma

Trizma® hydrochloride

BioPerformance Certified, cell culture tested, ≥99.0% (titration)

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride



Related Categories Apoptosis and Cell Cycle, BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts,
Quality Level   ELITE
grade   BioPerformance Certified
assay   ≥99.0% (titration)
impurities   DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
  endotoxin and total aerobic microbial count, tested
  ≤0.5% water (Karl Fischer)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble667 mg/mL
cation traces   heavy metals (as Pb): ≤5 ppm
absorption   A40%/290 ≤0.05
suitability   cell culture tested
  suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing


Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.


The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

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