|Related Categories||Apoptosis and Cell Cycle, BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts,|
|impurities||DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected|
|endotoxin and total aerobic microbial count, tested|
|≤0.5% water (Karl Fischer)|
|useful pH range||7.0 - 9.0|
|pKa (25 °C)||8.1|
|solubility||H2O: soluble667 mg/mL|
|cation traces||heavy metals (as Pb): ≤5 ppm|
|suitability||cell culture tested|
|suitable for electrophoresis|
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
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