Trizma® hydrochloride
for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)
DOWNLOAD MSDS (PDF)Synonym:
TRIS HCl,
TRIS hydrochloride,
Tris(hydroxymethyl)
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CAS Number 1185-53-1
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Linear Formula NH2C(CH2OH)3 · HCl
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Molecular Weight 157.60
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Beilstein Registry Number 3675235
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EC Number 214-684-5
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MDL number MFCD00012590
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PubChem Substance ID 24900332
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Popular Documents: Specification Sheet (PDF) | FTIR (PDF)
Properties
| Related Categories | Apoptosis and Cell Cycle, BioReagent Buffers, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions, |
| Quality Level | ELITE |
| grade | Biotechnology Performance Certified |
| for molecular biology | |
| assay | ≥99.0% (titration) |
| impurities | DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected |
| endotoxin and total aerobic microbial count, tested | |
| ≤0.5% water (Karl Fischer) |
Description
Other Notes
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
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Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)
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