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T5941 Sigma

Trizma® hydrochloride

BioPerformance Certified, cell culture tested, ≥99.0% (titration)

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride

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Properties

Related Categories Apoptosis and Cell Cycle, BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts,
Quality Level   ELITE
grade   BioPerformance Certified
assay   ≥99.0% (titration)
impurities   DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
  endotoxin and total aerobic microbial count, tested
  ≤0.5% water (Karl Fischer)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: soluble667 mg/mL
cation traces   heavy metals (as Pb): ≤5 ppm
absorption   A40%/290 ≤0.05
suitability   cell culture tested
  suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Interactive Buffer Table

New Lab Start-Up program
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

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