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T5944 Sigma

Tumor Necrosis Factor-α from rat

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym: Cachectin, TNF-α

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Properties

Related Categories Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Cytokines & Growth Factors, Cytokines Growth Factors and Hormones (Obesity),
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE and HPLC)
form   powder
quality   endotoxin tested
mol wt   predicted mol wt ~17 kDa
suitability   suitable for cell culture
storage temp.   −20°C

Description

Analysis Note

The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Physical form

Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier.

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Tumor Necrosis Factor (TNF) Superfamily

The tumor necrosis factor (TNF) superfamily comprise an increasing number of structurally related ligand-receptor pairs, including TNF-α, TNF-β (lymphotoxin, LT), TNFR1, TNFR2, LT-βR, osteoprotegrin ...
Jennifer Fries
BioFiles 2009, 4.5, 23.
Keywords: Apoptosis, Biofiles, Cell disruption, Ligands, Microscopy, Type

Peer-Reviewed Papers
15

References

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