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T5947 Sigma

Tissue protein, cell membrane

from rat kidney

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Properties

Related Categories Antibodies, Cell Biology, Cell Signaling and Neuroscience, Immunohistology and Blotting Controls, Signal Transduction Research Tools,
biological source   from rat kidney
form   liquid
shipped in   dry ice
storage temp.   −70°C

Description

Application

Highly purified cell membrane protein from whole tissue homogenates. Useful in electrophoresis, Western blotting, immunoassay, and other applications requiring source-specific, non-denatured protein.

Physical form

0.5 mg protein (approx. 3 mg protein/mL)

Supplied in a solution containing 20 mM HEPES, 1.5 mM MgCl2, 10 mM KCl, 40 mM sucrose, 1 mM EDTA, 2% glycerol, 0.5% sodium deoxycholate, 1% NP-40, pH 7.9, and a cocktail of protease inhibitors.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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