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T6025 Sigma

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

Synonym: TAE buffer

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z More...
InChI Key   HGEVZDLYZYVYHD-UHFFFAOYSA-N
sterility   0.2 μm filtered
product line   BioReagent
form   working solution
impurities   DNase, RNase, Protease, none detected
suitability   suitable for electrophoresis
  suitable for gel electrophoresis (after dilution to working concentration)
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Other Notes

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Price and Availability


Available in ELITE Grade
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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