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T6066 Sigma

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   ELITE
grade   BioPerformance Certified
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline powder
impurities   DNase, RNase, protease, none detected
  Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
  ≤0.2% water (Karl Fischer)
loss   ≤5% (loss on drying, 110 °C)
color   white
pH   10.0-11.5
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   water: soluble (666 mg/ml, yielding a clear colorless solution)
cation traces   heavy metals (as Pb): ≤2 ppm
absorption   A40%/290 ≤0.05
λ   (1M)
  (290nm A max < 0.05)
suitability   cell culture tested
  meets EP, USP testing specifications
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

Not tested for use in parenteral applications as specified in the EP monograph.

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tris Always Available
Protocols & Articles

Articles

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While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

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Buffer Reference Center

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Peer-Reviewed Papers
15

References

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