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T6146 Sigma

Trypan Blue

powder, BioReagent, suitable for cell culture

Synonym: Direct blue 14

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Properties

Related Categories Apoptosis and Cell Cycle, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
InChI Key   GLNADSQYFUSGOU-UHFFFAOYSA-J
product line   BioReagent
form   powder
composition   Dye content, ~40%
mp   >300 °C(lit.)
solubility   H2O: soluble10 mg/mL
suitability   suitable for cell culture
Gene Information   human ... PTPN1(5770), PTPRF(5792)

Description

Application

Use to detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability.

Packaging

5, 25, 100 g in glass bottle

Price and Availability


Filter out uncertainty.

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3
RTECS 
QJ6475000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Cell Viability and Proliferation

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents

Protocols

Cultrex® 3-D Spheroid Fluorometric Proliferation/Viability Assay Protocol

Current in vitro tumor models lack either a physiological context and/or reproducible format for evaluating tumor cells in vitro. At present, the most popular method for compound screening and pathwa...
Keywords: Cancer, Cell culture, Cell proliferation, Diagnostic, Diffusion, Gene expression, Genetic, Microscopy

Cultrex® 96-Well 3D Spheroid BME Cell Invasion Assay Protocol

The 96 Well 3D Spheroid BME Cell Invasion Assay offers a standardized, three dimensional, high content format for quantitating the degree to which invasive cells penetrate a barrier, consisting of ba...
Keywords: Adhesion, Cancer, Cell culture, Centrifugation, Diagnostic, Genetic, Microscopy, Polymerization reactions

Peer-Reviewed Papers
15

References

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