T6414 Sigma

Trypsin inhibitor from Glycine max (soybean)

solution, 1 ×, sterile-filtered, BioReagent, suitable for cell culture

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Synonym: SBTI

Description

Application

Optimized for passage of endothelial cell cultures.

Other Notes

Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.

View more information on Trypsin Inhibitor.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.

Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Biochem/physiol Actions

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.

General description

Soybean trypsin inhibitor exhibits a high degree of sequence homology with cathepsin D inhibitor from potatoes.1

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