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T6424 Sigma

Trypsin from human pancreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

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Description

Analysis Note

Protein determined by E1%/280.

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Quantity

Minimum 1,000 BAEE units/vial

Unit Definition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Price and Availability


Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YN5075000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Nα-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determina...

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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