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T6557 Sigma

Monoclonal Anti-γ-Tubulin antibody produced in mouse

clone GTU-88, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Centrosome,
species reactivity   Xenopus, canine, chicken, hamster, human, rat, bovine, mouse
application(s)   immunocytochemistry: 1:5,000-1:10,000 using HeLa cells
  indirect ELISA: suitable
  western blot: 1:10,000 using cultured chicken fibroblast extract
clone   GTU-88, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 48 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

synthetic γ-tubulin peptide (amino acids 38-53), conjugated to KLH.

General description

Gamma-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. Gamma-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. gamma-Tubulin abundance is less than 1% of the level of either α- or β-tubulin.

Specificity

Monoclonal Anti-gamma-Tubulin recognizes an epitope located in the N-terminal amino acids 38-53 of gamma-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus gamma-tubulin.

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Application

Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Price and Availability


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